Structural basis of biased T cell receptor recognition of an immunodominant HLA-A2 epitope of the SARS-CoV-2 spike protein

P Chaurasia; THO Nguyen; LC Rowntree; JA Juno; AK Wheatley; SJ Kent; K Kedzierska; J Rossjohn; J Petersen

Journal article

Structural basis of biased T cell receptor recognition of an immunodominant HLA-A2 epitope of the SARS-CoV-2 spike protein

P Chaurasia, THO Nguyen, LC Rowntree, JA Juno, AK Wheatley, SJ Kent, K Kedzierska, J Rossjohn, J Petersen

Journal of Biological Chemistry | ELSEVIER | Published : 2021

DOI: 10.1016/j.jbc.2021.101065

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Abstract

CD8+ T cells play an important role in vaccination and immunity against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Although numerous SARS-CoV-2 CD8+ T cell epitopes have been identified, the molecular basis underpinning T cell receptor (TCR) recognition of SARS-CoV-2-specific T cells remains unknown. The T cell response directed toward SARS-CoV-2 spike protein-derived S269-277 peptide presented by the human leukocyte antigen (HLA)A*02:01 allomorph (hereafter the HLA-A2S269-277 epitope) is, to date, the most immunodominant SARS-CoV-2 epitope found in individuals bearing this allele. As HLA-A2S269-277 - specific CD8+ T cells utilize biased TRAV12 gene usage within ..

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University of Melbourne Researchers

Oanh Nguyen Author

Louise Rowntree Author

Jennifer Juno Author

Adam Wheatley Author

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Grants

Awarded by Australian Research Council

Funding Acknowledgements

This work was supported by the NHMRC Leadership Investigator Grant to K. K. (1173871), NHMRC Emerging Leadership Level 1 Investigator Grant to T. H. O. N. (#1194036), Research Grants Council of the Hong Kong Special Administrative Region, China (#T11-712/19-N) to K. K., and MRFF Award (#2005544) to K. K., S. J. K., A. K. W., and J. A. J. A. K. W. are supported by Emerging Leadership 1 Investigator Grant (#1173433), J. A. J. by an NHMRC Early Career Fellowship (ECF) (#1123673), and S. J. K. by NHMRC Senior Principal Research Fellowship (#1136322). J. R. is supported by an ARC Laureate fellowship.